Poster Session: 11:00AM - 1:00PM ET

Development of a novel microglial specific marker antibody: Anti P2RY12 guinea pig polyclonal antibody

PRESENTER:  

Dr. Naomi Tsurutani | Sales Account Manager, Lab Chemicals East

PRESENTER'S BIO: 

Naomi Tsurutani received her Ph.D. in Virology/Immunology from Tokyo Medical and Dental University in Tokyo, Japan. Before joining FUJIFILM Wako, she worked as an immunology researcher at Emory University, UMass Medical School, and UConn Health. 

At FUJIFILM Wako, she helps researchers better perform and communicate their research using FUJIFILM Wako products.

ABSTRACT:

Microglia are the primary resident immune cells in brain parenchyma and play a variety of roles, such as releasing cytokines and phagocytosis of foreign and dead cells. The heterogeneity of microglial populations and the concept of disease-associated microglia have been proposed, and their relationship to neurological diseases has been actively investigated. On the other hand, macrophages have recently been reported to be resident in specific areas of the central nervous system (CNS). These macrophages, called CNS-associated macrophages (CAMs), are found in meninges and perivascular spaces, and their properties are very similar to those of microglia. To understand the true microglial dynamics and how they are causally related to CNS disease, it is critical to accurately distinguish microglia from macrophages. Purinergic receptor P2Y, G-protein coupled 12 (P2RY12) and Transmembrane Protein 119 (TMEM119) are currently used as markers to discriminate between microglia and macrophages. However, commercially available microglial specific marker antibodies are confined to a limited number of animal species and lack versatility in multiple staining. In this study, we set out to develop a guinea pig derived anti-P2RY12 antibody with high versatility, specificity and stable performance in immunohistochemistry.