LIVE Webinar - Available 11:30AM - 12:00PM ET

Development, Characterization and Analysis of High-Throughput Spheroids for Cancer Research

PRESENTER: 

Dr. Isha Dey | R&D Scientist at Thermo Fisher Scientific 

PRESENTER'S BIO: 

Isha Dey received her Ph.D. in Physiology and Biophysics from Rosalind Franklin University of Medicine and Science at the Chicago Medical School. Since joining Thermo Fisher Scientific in 2018, she has worked on developing 3D models for cancer studies, physiological disease models as well as high throughput imaging and analysis of these models. She does application work to support portfolio development and marketability for new and legacy products.

ABSTRACT:

Spheroids and organoids, two examples of 3D cell culture models, have become invaluable tools to study the processes that dictate behavior of tissues under physiological and pathological conditions, both in basic and pre-clinical research. But challenge lies in the robust generation of uniform spheroids to minimize data variation, increase reproducibility of 3D-based high-throughput assays, and therefore yield useful data. In this webinar, we will provide guidelines for making 3D cell models in-vitro, with emphasis on high-throughput generation and culturing of spheroids. We will also highlight methods and tools to characterize the spheroids to respect to size, compactness, cell viability and response to cytotoxic drugs.